AIM2 Monoclonal antibody

AIM2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4C6E1

Cat no : 66902-3-Ig

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Synonyms

PYHIN4, Interferon-inducible protein AIM2, 4C6E1



经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Karpas-299 cells, U266 cells, MJ cells, HuT 78 cells, U-87 MG cells
Planning an IHC experiment? We recommend our IHCeasy AIM2 Ready-To-Use IHC Kit. AIM2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66902-3-Ig targets AIM2 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen AIM2 fusion protein Ag14607 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name absent in melanoma 2
Synonyms PYHIN4, Interferon-inducible protein AIM2, 4C6E1
Calculated Molecular Weight 343 aa, 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC010940
Gene Symbol AIM2
Gene ID (NCBI) 9447
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14862
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]

实验方案

Product Specific Protocols
WB protocol for AIM2 antibody 66902-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols