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MAVS; VISA Monoclonal antibody, PBS Only (Capture)

MAVS; VISA Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A8E9

Cat no : 66911-1-PBS

Print datasheet

Synonyms

MAVS, KIAA1271, IPS-1, IPS1, IPS 1

验证数据展示

  • WB analysis of A431 using 66911-1-Ig (same clone as 66911-1-PBS)

    WB analysis of A431 using 66911-1-Ig (same clone as 66911-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

  • WB analysis using 66911-1-Ig (same clone as 66911-1-PBS)

    WB analysis using 66911-1-Ig (same clone as 66911-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66911-1-Ig (same clone as 66911-1-PBS)

    IHC staining of human liver cancer using 66911-1-Ig (same clone as 66911-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66911-1-Ig (same clone as 66911-1-PBS)

    IHC staining of human liver cancer using 66911-1-Ig (same clone as 66911-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50509-3

    Cytometric bead array standard curve of MP50509-3

    Cytometric bead array standard curve of MP50509-3, MAVS; VISA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66911-1-PBS. Detection antibody: 66911-4-PBS. Standard:Ag5949. Range: 0.391-50 ng/mL.

产品信息

66911-1-PBS targets MAVS; VISA as part of a matched antibody pair:

MP50509-3: 66911-1-PBS capture and 66911-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen MAVS; VISA fusion protein Ag5949 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mitochondrial antiviral signaling protein
Synonyms MAVS, KIAA1271, IPS-1, IPS1, IPS 1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight50-55 kDa, 70-75 kDa
GenBank Accession NumberBC044952
Gene Symbol MAVS
Gene ID (NCBI) 57506
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7Z434
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.