验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, Jurkat cells, L02 cells, HEK-293 cells, THP-1cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:550-1:2200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66911-1-Ig targets MAVS; VISA in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IF, IP, CoIP |
经测试反应性 | human |
文献引用反应性 | human, mouse, pig, monkey |
免疫原 | MAVS; VISA fusion protein Ag5949 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | mitochondrial antiviral signaling protein |
别名 | MAVS, KIAA1271, IPS-1, IPS1, IPS 1 |
计算分子量 | 57 kDa |
观测分子量 | 50-55 kDa, 70-75 kDa |
GenBank蛋白编号 | BC044952 |
基因名称 | MAVS |
Gene ID (NCBI) | 57506 |
RRID | AB_2882238 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q7Z434 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for MAVS; VISA antibody 66911-1-Ig | Download protocol |
IHC protocol for MAVS; VISA antibody 66911-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation | ||
J Immunother Cancer Targeting KDM4C enhances CD8+ T cell mediated antitumor immunity by activating chemokine CXCL10 transcription in lung cancer. | ||
Front Immunol Swine Acute Diarrhea Syndrome Coronavirus Nucleocapsid Protein Antagonizes Interferon-β Production via Blocking the Interaction Between TRAF3 and TBK1. | ||
J Virol Encephalomyocarditis virus abrogates the IFN-β signaling pathway via its structural protein VP2. | ||
Vet Microbiol DDX56 antagonizes IFN-β production to enhance EMCV replication by inhibiting IRF3 nuclear translocation | ||
Virus Res Host protein, HSP90β, antagonizes IFN-β signaling pathway and facilitates the proliferation of encephalomyocarditis virus in vitro. |