• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MAVS; VISA Monoclonal antibody, PBS Only

MAVS; VISA Monoclonal Antibody for WB,Indirect ELISA,IHC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,Indirect ELISA,IHC

Conjugate

Unconjugated

CloneNo.

1A8E9

Cat No : 66911-1-PBS

Print datasheet

Synonyms

CARDIF, DKFZp547C224, DKFZp666M015, FLJ27482, FLJ35386, FLJ38051, FLJ41962, IPS 1, IPS1, KIAA1271, MAVS, MAVS; VISA, VISA

验证数据展示

  • WB analysis of A431 using 66911-1-Ig (same clone as 66911-1-PBS)

    WB analysis of A431 using 66911-1-Ig (same clone as 66911-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

  • WB analysis using 66911-1-Ig (same clone as 66911-1-PBS)

    WB analysis using 66911-1-Ig (same clone as 66911-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66911-1-Ig (same clone as 66911-1-PBS)

    IHC staining of human liver cancer using 66911-1-Ig (same clone as 66911-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66911-1-Ig (same clone as 66911-1-PBS)

    IHC staining of human liver cancer using 66911-1-Ig (same clone as 66911-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

产品信息

66911-1-PBS targets MAVS; VISA in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IHC Application Description
Tested Reactivity Human
Immunogen MAVS; VISA fusion protein Ag5949 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mitochondrial antiviral signaling protein
Synonyms CARDIF, DKFZp547C224, DKFZp666M015, FLJ27482, FLJ35386, FLJ38051, FLJ41962, IPS 1, IPS1, KIAA1271, MAVS, MAVS; VISA, VISA
Calculated Molecular Weight 57 kDa
Observed Molecular Weight50-55 kDa, 70-75 kDa
GenBank Accession NumberBC044952
Gene Symbol MAVS
Gene ID (NCBI) 57506
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7Z434
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.