验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, HEK-293 cells, NCI-H1299 cells |
Planning an IHC experiment? We recommend our IHCeasy MMP12 Ready-To-Use IHC Kit. MMP12 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 2 publications below |
产品信息
66930-1-Ig targets MMP12 in WB, IHC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | Human |
| 文献引用反应性 | human, mouse |
| 免疫原 | MMP12 fusion protein Ag19068 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | matrix metallopeptidase 12 (macrophage elastase) |
| 别名 | HME, Macrophage elastase, Macrophage metalloelastase, Matrix metalloproteinase 12, ME, MME, MMP 12, MMP12 |
| 计算分子量 | 470 aa, 54 kDa |
| 观测分子量 | 54 kDa, 45 kDa |
| GenBank蛋白编号 | BC112301 |
| 基因名称 | MMP12 |
| Gene ID (NCBI) | 4321 |
| RRID | AB_2882256 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P39900 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MMP12(Matrix metalloproteinase-12) is a 54 kDa proenzyme that is processed into a 45 kDa and then a 22 kDa active form(15723202). MMP9 and MMP12 promote intimal thickening by independent cleavage of N-cadherin, which elevates vascular smooth muscle cell proliferation via beta-catenin signalling. Its overexpression in myeloid lineage cells plays a key role in modulating myelopoiesis, immune suppression, and lung tumorigenesis(PMID: 21378275).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MMP12 antibody 66930-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Eur J Pharmacol Reversal of elastase-induced abdominal aortic aneurysm following the delivery of nanoparticle-based pentagalloyl glucose (PGG) is associated with reduced inflammatory and immune markers. | ||
Drug Des Devel Ther Identification of Active Compounds and Mechanism of Huangtu Decoction for the Treatment of Ulcerative Colitis by Network Pharmacology Combined with Experimental Verification | ||
Cytokine IL-33/ST2 enhances MMP-12 expression by macrophages to mediate inflammatory and immune response in IgG4-Related Ophthalmic Disease |
