验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, THP-1 cells, HSC-T6 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
66932-2-Ig targets ARH in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human |
| 免疫原 | ARH fusion protein Ag28608 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | low density lipoprotein receptor adaptor protein 1 |
| 别名 | LDLRAP1, FHCB2, FHCB1, ARH2, ARH1 |
| 计算分子量 | 308 aa, 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC029770 |
| 基因名称 | ARH |
| Gene ID (NCBI) | 26119 |
| RRID | AB_2882258 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q5SW96 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARH antibody 66932-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
