验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, rat lung tissue, pig lung tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
66934-1-Ig targets PHEX in WB, IHC, ELISA applications and shows reactivity with Human, Rat, Pig samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, Rat, Pig |
| 文献引用反应性 | human, rat |
| 免疫原 | PHEX fusion protein Ag20290 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | phosphate regulating endopeptidase homolog, X-linked |
| 别名 | HPDR, HPDR1, HYP, HYP1, PHEX, XLH |
| 计算分子量 | 749 aa, 86 kDa |
| 观测分子量 | 86-100 kDa |
| GenBank蛋白编号 | BC105059 |
| 基因名称 | PHEX |
| Gene ID (NCBI) | 5251 |
| RRID | AB_2882259 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P78562 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PHEX encodes a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein usually expressed in osteocytes, osteoblasts and odontoblasts, and it was thought to be involved in bone and dentin mineralization and renal phosphate reabsorption (PMID:28982589). Otherwise, studies showed that PHEX transcripts were identified in human osteosarcoma-derived cells and in differentiated mouse osteoblasts, but not in immature mouse preosteoblasts, indicating the stage-specific expression of the protein (PMID:9199999). Defects in PHEX are responsible for X-linked hypophosphatemic rickets (HYP) (PMID:22339660). Glycosylation of proteins results in higher molecular weight, and about 95-100 kDa band were reported (PMID:25128529; PMID:18728349).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PHEX antibody 66934-1-Ig | Download protocol |
| IHC protocol for PHEX antibody 66934-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Biomater Osteoinductivity and biomechanical assessment of a 3D printed demineralized bone matrix-ceramic composite in a rat spine fusion model. | ||
J Clin Endocrinol Metab Characterization of novel PHEX variants in X-linked hypophosphatemic rickets and genotype-PHEX activity correlation |
