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PHEX Monoclonal antibody, PBS Only

PHEX Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D4B2

Cat no : 66934-1-PBS

Print datasheet

Synonyms

HPDR, HPDR1, HYP, HYP1, PHEX, XLH

验证数据展示

  • WB analysis of HEK-293 using 66934-1-Ig (same clone as 66934-1-PBS)

    WB analysis of HEK-293 using 66934-1-Ig (same clone as 66934-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66934-1-Ig (PHEX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66934-1-Ig (same clone as 66934-1-PBS)

    IHC staining of human lung cancer using 66934-1-Ig (same clone as 66934-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66934-1-Ig (PHEX antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66934-1-PBS in a different storage buffer formulation.

产品信息

66934-1-PBS targets PHEX in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Pig samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Rat, Pig
Immunogen PHEX fusion protein Ag20290 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphate regulating endopeptidase homolog, X-linked
Synonyms HPDR, HPDR1, HYP, HYP1, PHEX, XLH
Calculated Molecular Weight 749 aa, 86 kDa
Observed Molecular Weight 86-100 kDa
GenBank Accession NumberBC105059
Gene Symbol PHEX
Gene ID (NCBI) 5251
RRIDAB_2882259
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78562
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PHEX encodes a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein usually expressed in osteocytes, osteoblasts and odontoblasts, and it was thought to be involved in bone and dentin mineralization and renal phosphate reabsorption (PMID:28982589). Otherwise, studies showed that PHEX transcripts were identified in human osteosarcoma-derived cells and in differentiated mouse osteoblasts, but not in immature mouse preosteoblasts, indicating the stage-specific expression of the protein (PMID:9199999). Defects in PHEX are responsible for X-linked hypophosphatemic rickets (HYP) (PMID:22339660).Glycosylation of proteins results in higher molecular weight, and about 95-100 kDa band were reported (PMID:25128529; PMID:18728349).