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CEP63 Monoclonal antibody

CEP63 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G12B2

Cat no : 66996-1-Ig

Print datasheet

Synonyms

centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63, FLJ13386

验证数据展示

  • WB analysis using 66996-1-Ig

    WB analysis using 66996-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66996-1-Ig (CEP63 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 66996-1-Ig

    IF Staining of hTERT-RPE1 using 66996-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CEP63 antibody (66996-1-Ig, Clone: 2G12B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, T-47D cells, PC-3 cells, HepG2 cells, HSC-T6 cells
Positive IF/ICC detected inhTERT-RPE1 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66996-1-Ig targets CEP63 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen CEP63 fusion protein Ag9227 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name centrosomal protein 63kDa
Synonyms centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63, FLJ13386
Calculated Molecular Weight 703 aa, 81 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC014050
Gene Symbol CEP63
Gene ID (NCBI) 80254
RRIDAB_2882313
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96MT8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP63 is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response.

实验方案

Product Specific Protocols
WB protocol for CEP63 antibody 66996-1-IgDownload protocol
IF protocol for CEP63 antibody 66996-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols