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CoraLite® Plus 488-conjugated CEP63 Monoclonal antibody

CEP63 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2G12B2

Cat no : CL488-66996

Print datasheet

Synonyms

centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63, FLJ13386

验证数据展示

  • IF Staining of hTERT-RPE1 using CL488-66996

    IF Staining of hTERT-RPE1 using CL488-66996

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite® Plus 488 CEP63 antibody (CL488-66996, Clone: 2G12B2 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells
For other applications, we recommend the unconjugated version of this antibody, 66996-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66996 targets CEP63 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen CEP63 fusion protein Ag9227 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name centrosomal protein 63kDa
Synonyms centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63, FLJ13386
Calculated Molecular Weight 703 aa, 81 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC014050
Gene Symbol CEP63
Gene ID (NCBI) 80254
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96MT8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP63 is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CEP63 antibody CL488-66996Download protocol
Standard Protocols
Click here to view our Standard Protocols