验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, MCF-7 cells, T-47D cells, PC-3 cells, HepG2 cells, HSC-T6 cells |
Positive IF/ICC detected in | hTERT-RPE1 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66996-1-Ig targets CEP63 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | Human, Mouse, Rat |
免疫原 | CEP63 fusion protein Ag9227 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | centrosomal protein 63kDa |
别名 | centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63, FLJ13386 |
计算分子量 | 703 aa, 81 kDa |
观测分子量 | 85 kDa |
GenBank蛋白编号 | BC014050 |
基因名称 | CEP63 |
Gene ID (NCBI) | 80254 |
RRID | AB_2882313 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q96MT8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP63 is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response.
实验方案
Product Specific Protocols | |
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WB protocol for CEP63 antibody 66996-1-Ig | Download protocol |
IF protocol for CEP63 antibody 66996-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |