验证数据展示
经过测试的应用
| Positive WB detected in | pig cerebellum tissue, pig brain tissue, rat brain tissue, rat cerebellum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67010-1-Ig targets RASGRF1 in WB, ELISA applications and shows reactivity with rat, pig, Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | rat, pig, Human |
| 免疫原 | RASGRF1 fusion protein Ag6937 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Ras protein-specific guanine nucleotide-releasing factor 1 |
| 别名 | CDC25, CDC25L, GNRP, GRF1, GRF55, H GRF55, PP13187, Ras GRF1, RAS-GRF, RASGRF1 |
| 计算分子量 | 134 kDa |
| 观测分子量 | 145 kDa |
| GenBank蛋白编号 | BC040275 |
| 基因名称 | RASGRF1 |
| Gene ID (NCBI) | 5923 |
| RRID | AB_2882327 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13972 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RasGRF1, a member of calmodulin-activated guanine-nucleotide exchange factors (GEFs), highly expressed at the synaptic junctions of the central nervous system (CNS). It is similar to the Saccharomyces cerevisiae CDC25. RASGRF1 serves as an in vivo activator for H-RAS and members of the R-RAS and RAC subfamilies. Several studies have shown that RasGRF1 also binds to microtubules and participate in the regulation of neurite outgrowth. RasGRF1 has been implicated in neuronal excitability. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RASGRF1 antibody 67010-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
