RASGRF1 Monoclonal antibody
RASGRF1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
rat, pig, Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1A4D4
验证数据展示
经过测试的应用
| Positive WB detected in | pig cerebellum tissue, pig brain tissue, rat brain tissue, rat cerebellum tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67010-1-Ig targets RASGRF1 in WB, ELISA applications and shows reactivity with rat, pig, Human samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | rat, pig, Human |
| Immunogen | RASGRF1 fusion protein Ag6937 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | Ras protein-specific guanine nucleotide-releasing factor 1 |
| Synonyms | CDC25, CDC25L, GNRP, GRF1, GRF55, H GRF55, PP13187, Ras GRF1, RAS-GRF, RASGRF1 |
| Calculated Molecular Weight | 134 kDa |
| Observed Molecular Weight | 145 kDa |
| GenBank Accession Number | BC040275 |
| Gene Symbol | RASGRF1 |
| Gene ID (NCBI) | 5923 |
| RRID | AB_2882327 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13972 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RasGRF1, a member of calmodulin-activated guanine-nucleotide exchange factors (GEFs), highly expressed at the synaptic junctions of the central nervous system (CNS). It is similar to the Saccharomyces cerevisiae CDC25. RASGRF1 serves as an in vivo activator for H-RAS and members of the R-RAS and RAC subfamilies. Several studies have shown that RasGRF1 also binds to microtubules and participate in the regulation of neurite outgrowth. RasGRF1 has been implicated in neuronal excitability. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RASGRF1 antibody 67010-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
