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RASGRF1 Monoclonal antibody, PBS Only

RASGRF1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rat, pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A4D4

Cat no : 67010-1-PBS

Print datasheet

Synonyms

CDC25, CDC25L, GNRP, GRF1, GRF55, H GRF55, PP13187, Ras GRF1, RAS-GRF, RASGRF1

验证数据展示

  • WB analysis of pig cerebellum using 67010-1-Ig (same clone as 67010-1-PBS)

    WB analysis of pig cerebellum using 67010-1-Ig (same clone as 67010-1-PBS)

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67010-1-Ig (RASGRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67010-1-PBS in a different storage buffer formulation.

产品信息

67010-1-PBS targets RASGRF1 in WB, Indirect ELISA applications and shows reactivity with rat, pig samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity rat, pig
Immunogen RASGRF1 fusion protein Ag6937 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Ras protein-specific guanine nucleotide-releasing factor 1
Synonyms CDC25, CDC25L, GNRP, GRF1, GRF55, H GRF55, PP13187, Ras GRF1, RAS-GRF, RASGRF1
Calculated Molecular Weight 134 kDa
Observed Molecular Weight 145 kDa
GenBank Accession NumberBC040275
Gene Symbol RASGRF1
Gene ID (NCBI) 5923
RRIDAB_2882327
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13972
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RasGRF1, a member of calmodulin-activated guanine-nucleotide exchange factors (GEFs), highly expressed at the synaptic junctions of the central nervous system (CNS). It is similar to the Saccharomyces cerevisiae CDC25. RASGRF1 serves as an in vivo activator for H-RAS and members of the R-RAS and RAC subfamilies. Several studies have shown that RasGRF1 also binds to microtubules and participate in the regulation of neurite outgrowth. RasGRF1 has been implicated in neuronal excitability. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.