验证数据展示
经过测试的应用
| Positive WB detected in | HSC-T6 cells, HepG2 cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67021-1-Ig targets NFS1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | NFS1 fusion protein Ag8017 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | NFS1 nitrogen fixation 1 homolog (S. cerevisiae) |
| 别名 | IscS, HUSSY-08, HUSSY 08, EC:2.8.1.7, Cysteine desulfurase |
| 计算分子量 | 50 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC018471 |
| 基因名称 | NFS1 |
| Gene ID (NCBI) | 9054 |
| RRID | AB_2918480 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9Y697 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NFS1 antibody 67021-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
