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  • Featured Product
  • KD/KO Validated

NFS1 Monoclonal antibody, PBS Only

NFS1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A10A7

Cat no : 67021-1-PBS

Print datasheet

Synonyms

HUSSY 08, IscS, NFS1, NIFS

验证数据展示

  • WB analysis of HSC-T6 using 67021-1-Ig (same clone as 67021-1-PBS)

    WB analysis of HSC-T6 using 67021-1-Ig (same clone as 67021-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67021-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 67021-1-Ig (same clone as 67021-1-PBS)

    WB analysis of HepG2 using 67021-1-Ig (same clone as 67021-1-PBS)

    WB result of NFS1 antibody (67021-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NFS1 transfected HepG2 cells. This data was developed using the same antibody clone with 67021-1-PBS in a different storage buffer formulation.

产品信息

67021-1-PBS targets NFS1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen NFS1 fusion protein Ag8017 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
Synonyms HUSSY 08, IscS, NFS1, NIFS
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC018471
Gene Symbol NFS1
Gene ID (NCBI) 9054
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y697
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.