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MLX Monoclonal antibody

MLX Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A8D2

Cat no : 67042-1-Ig

Print datasheet

Synonyms

bHLHd13, MAD7, Max like bHLHZip protein, MAX like protein X, MLX, MXD7, Protein BigMax, TCFL4

验证数据展示

  • WB analysis using 67042-1-Ig

    WB analysis using 67042-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67042-1-Ig (MLX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67042-1-Ig

    IHC staining of human breast cancer using 67042-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MLX antibody included
  • IF Staining of HepG2 using 67042-1-Ig

    IF Staining of HepG2 using 67042-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MLX antibody (67042-1-Ig, Clone: 2A8D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue).

  • IF Staining of HepG2 using 67042-1-Ig

    IF Staining of HepG2 using 67042-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MLX antibody (67042-1-Ig, Clone: 2A8D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MLX Ready-To-Use IHC Kit. MLX primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67042-1-Ig targets MLX in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen MLX fusion protein Ag28729 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name MAX-like protein X
Synonyms bHLHd13, MAD7, Max like bHLHZip protein, MAX like protein X, MLX, MXD7, Protein BigMax, TCFL4
Calculated Molecular Weight 298 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC010689
Gene Symbol MLX
Gene ID (NCBI) 6945
RRIDAB_2882356
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UH92
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MLX antibody 67042-1-IgDownload protocol
IHC protocol for MLX antibody 67042-1-IgDownload protocol
IF protocol for MLX antibody 67042-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols