验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, NIH/3T3 cells, U2OS cells, MCF-7 cells, Caco-2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
产品信息
67048-1-Ig targets Cyclin D2 in WB, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | Cyclin D2 fusion protein Ag25190 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cyclin D2 |
| 别名 | CCND2, cyclin D, cyclin D2, G1/S specific cyclin D2, KIAK0002 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC010958 |
| 基因名称 | Cyclin D2 |
| Gene ID (NCBI) | 894 |
| RRID | AB_2882361 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P30279 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. CCND2 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of CCND2 was observed in ovarian and testicular tumors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cyclin D2 antibody 67048-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation | ||
Technol Cancer Res Treat The Analysis of Differentially Expressed circRNAs Under the Antiproliferative Effect From 5-Fluorouracil on Osteosarcoma Cells. | ||
Cell Death Dis Long intergenic non-coding RNA 00473 promotes proliferation and migration of gastric cancer via the miR-16-5p/CCND2 axis and by regulating AQP3. | ||
Am J Cancer Res miR-1297 inhibits osteosarcoma cell proliferation and growth by targeting CCND2 | ||
Int Immunopharmacol Hydroxypropyl-β-cyclodextrin inhibits the development of triple negative breast cancer by enhancing antitumor immunity | ||
Cancer Chemother Pharmacol Avermectin B1 mediates antitumor activity and induces autophagy in osteosarcoma through the AMPK/ULK1 signaling pathway |
