PELP1 Monoclonal antibody
PELP1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human and More (1)
Applications
WB, IHC, IF-P, FC (Intra), ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1B11E4
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, T-47D cells, Jurkat cells, HEK-293 cells, MCF-7 cells |
Positive IHC detected in | human cervical cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human breast cancer tissue |
Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67050-1-Ig targets PELP1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human |
Cited Reactivity | mouse |
Immunogen | PELP1 fusion protein Ag25729 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | proline, glutamate and leucine rich protein 1 |
Synonyms | Transcription factor HMX3, Proline-, glutamic acid- and leucine-rich protein 1, Modulator of non-genomic activity of estrogen receptor, MNAR, HMX3 |
Calculated Molecular Weight | 1130 aa, 120 kDa |
Observed Molecular Weight | 160 kDa |
GenBank Accession Number | BC069058 |
Gene Symbol | PELP1 |
Gene ID (NCBI) | 27043 |
RRID | AB_2882363 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q8IZL8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PELP1 antibody 67050-1-Ig | Download protocol |
IHC protocol for PELP1 antibody 67050-1-Ig | Download protocol |
IF protocol for PELP1 antibody 67050-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Biotechnol MiR-153 is Involved in Testicular Ischemia/Reperfusion Injury by Directly Targeting FOXO3 and Regulating Spermatogonia Pyroptosis | ||
Int Immunopharmacol MiR-195-5p is involved in testicular ischemia/reperfusion injury by directly targeting PELP1 and regulating spermatogonia pyroptosis |