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RALB Monoclonal antibody, PBS Only (Detector)

RALB Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, pig, mouse

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D11C11

Cat no : 67094-1-PBS

Print datasheet

Synonyms

RALB, Ras related protein Ral B

验证数据展示

  • WB analysis using 67094-1-Ig (same clone as 67094-1-PBS)

    WB analysis using 67094-1-Ig (same clone as 67094-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67094-1-Ig (RALB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • WB analysis using 67094-1-Ig (same clone as 67094-1-PBS)

    WB analysis using 67094-1-Ig (same clone as 67094-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67094-1-Ig (RALB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • IF Staining of human lung cancer using 67094-1-Ig (same clone as 67094-1-PBS)

    IF Staining of human lung cancer using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using RALB antibody (67094-1-Ig, Clone: 1D11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse bladder using 67094-1-Ig (same clone as 67094-1-PBS)

    IHC staining of mouse bladder using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of mouse placenta using 67094-1-Ig (same clone as 67094-1-PBS)

    IHC staining of mouse placenta using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of mouse liver using 67094-1-Ig (same clone as 67094-1-PBS)

    IHC staining of mouse liver using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of mouse lung using 67094-1-Ig (same clone as 67094-1-PBS)

    IHC staining of mouse lung using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of human lung cancer using 67094-1-Ig (same clone as 67094-1-PBS)

    IHC staining of human lung cancer using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RALB antibody included
  • Cytometric bead array standard curve of MP50129-1

    Cytometric bead array standard curve of MP50129-1

    Cytometric bead array standard curve of MP50129-1, RALB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67094-2-PBS. Detection antibody: 67094-1-PBS. Standard:Ag28600. Range: 6.25-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy RALB Ready-To-Use IHC Kit. RALB primary antibody included.

产品信息

67094-1-PBS targets RALB as part of a matched antibody pair:

MP50129-1: 67094-2-PBS capture and 67094-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity Human, rat, pig, mouse
Immunogen RALB fusion protein Ag28600 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Affinity
KD=1.82 x 10-10M
KOff=2.54 x 10-6M
KOn=1.40 x 104M
Full Name v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
Synonyms RALB, Ras related protein Ral B
Calculated Molecular Weight 206 aa, 23 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC018163
Gene Symbol RALB
Gene ID (NCBI) 5899
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11234
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C