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验证数据展示

  • WB analysis using 67094-1-Ig (same clone as 67094-1-PBS)

    WB analysis using 67094-1-Ig (same clone as 67094-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67094-1-Ig (RALB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • WB analysis using 67094-1-Ig (same clone as 67094-1-PBS)

    WB analysis using 67094-1-Ig (same clone as 67094-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67094-1-Ig (RALB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • IF Staining of human lung cancer using 67094-1-Ig (same clone as 67094-1-PBS)

    IF Staining of human lung cancer using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using RALB antibody (67094-1-Ig, Clone: 1D11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse bladder using 67094-1-Ig (same clone as 67094-1-PBS)

    IHC staining of mouse bladder using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of mouse placenta using 67094-1-Ig (same clone as 67094-1-PBS)

    IHC staining of mouse placenta using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of mouse liver using 67094-1-Ig (same clone as 67094-1-PBS)

    IHC staining of mouse liver using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of mouse lung using 67094-1-Ig (same clone as 67094-1-PBS)

    IHC staining of mouse lung using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of human lung cancer using 67094-1-Ig (same clone as 67094-1-PBS)

    IHC staining of human lung cancer using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RALB antibody included
  • Cytometric bead array standard curve of MP50129-1

    Cytometric bead array standard curve of MP50129-1

    Cytometric bead array standard curve of MP50129-1, RALB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67094-2-PBS. Detection antibody: 67094-1-PBS. Standard:Ag28600. Range: 6.25-100 ng/mL.

RALB Monoclonal antibody, PBS Only (Detector)

RALB Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 67094-1-PBS

产品说明书

CloneNo. 1D11C11

宿主/亚型

Mouse / IgG2a

种属反应性

Human, rat, pig, mouse

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

RALB, Ras related protein Ral B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RALB Ready-To-Use IHC Kit. RALB primary antibody included.

产品信息

67094-1-PBS targets RALB as part of a matched antibody pair:

MP50129-1: 67094-2-PBS capture and 67094-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 Human, rat, pig, mouse
免疫原 RALB fusion protein Ag28600 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
亲和力
KD=1.82 x 10-10M
KOff=2.54 x 10-6M
KOn=1.40 x 104M
全称 v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
别名 RALB, Ras related protein Ral B
计算分子量 206 aa, 23 kDa
观测分子量 24 kDa
GenBank蛋白编号BC018163
基因名称 RALB
Gene ID (NCBI) 5899
RRIDAB_2882399
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11234
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C