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RALB Monoclonal antibody

RALB Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, pig, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1D11C11

Cat no : 67094-1-Ig

Print datasheet

Synonyms

RALB, Ras related protein Ral B

验证数据展示

  • WB analysis using 67094-1-Ig

    WB analysis using 67094-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67094-1-Ig (RALB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67094-1-Ig

    WB analysis using 67094-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67094-1-Ig (RALB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of human lung cancer using 67094-1-Ig

    IF Staining of human lung cancer using 67094-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using RALB antibody (67094-1-Ig, Clone: 1D11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of mouse bladder using 67094-1-Ig

    IHC staining of mouse bladder using 67094-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of mouse placenta using 67094-1-Ig

    IHC staining of mouse placenta using 67094-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of mouse liver using 67094-1-Ig

    IHC staining of mouse liver using 67094-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of mouse lung using 67094-1-Ig

    IHC staining of mouse lung using 67094-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of human lung cancer using 67094-1-Ig

    IHC staining of human lung cancer using 67094-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RALB antibody included

经过测试的应用

Positive WB detected inHeLa cells, rat brain tissue, mouse brain tissue, MCF-7 cells, HepG2 cells, Jurkat cells, Pig brain cells
Positive IHC detected inmouse bladder tissue, human lung cancer tissue, mouse liver tissue, mouse lung tissue, mouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue
Planning an IHC experiment? We recommend our IHCeasy RALB Ready-To-Use IHC Kit. RALB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67094-1-Ig targets RALB in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, rat, pig, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsIF, IHC
Tested Reactivity Human, rat, pig, mouse
Cited Reactivityhuman
Immunogen RALB fusion protein Ag28600 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Affinity
KD=1.82 x 10-10M
KOff=2.54 x 10-6M
KOn=1.40 x 104M
Full Name v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
Synonyms RALB, Ras related protein Ral B
Calculated Molecular Weight 206 aa, 23 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC018163
Gene Symbol RALB
Gene ID (NCBI) 5899
RRIDAB_2882399
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11234
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RALB antibody 67094-1-IgDownload protocol
IHC protocol for RALB antibody 67094-1-IgDownload protocol
IF protocol for RALB antibody 67094-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Hum Pathol

Papillary renal neoplasm with reverse polarity: a clinicopathologic study of 43 cases with a focus on the expression of KRAS signaling pathway downstream effectors

Authors - Bohyun Kim
View Article
humanIF

Cancers (Basel)

Controlled Plasma Membrane Delivery of FGFR1 and Modulation of Signaling by a Novel Regulated Anterograde RTK Transport Pathway

Authors - Claire Leist Hinsch
View Article