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RALB Monoclonal antibody, PBS Only (Detector)

RALB Monoclonal Antibody for WB, IF, IHC, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, pig, mouse

Applications

WB, IF, IHC, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

1D11C11

Cat No : 67094-1-PBS

Print datasheet

Synonyms

RALB, Ras related protein Ral B

验证数据展示

  • WB analysis using 67094-1-Ig (same clone as 67094-1-PBS)

    WB analysis using 67094-1-Ig (same clone as 67094-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67094-1-Ig (RALB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • WB analysis using 67094-1-Ig (same clone as 67094-1-PBS)

    WB analysis using 67094-1-Ig (same clone as 67094-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67094-1-Ig (RALB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • IF Staining of human lung cancer using 67094-1-Ig (same clone as 67094-1-PBS)

    IF Staining of human lung cancer using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using RALB antibody (67094-1-Ig, Clone: 1D11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse bladder using 67094-1-Ig (same clone as 67094-1-PBS)

    IHC staining of mouse bladder using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of mouse placenta using 67094-1-Ig (same clone as 67094-1-PBS)

    IHC staining of mouse placenta using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of mouse liver using 67094-1-Ig (same clone as 67094-1-PBS)

    IHC staining of mouse liver using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of mouse lung using 67094-1-Ig (same clone as 67094-1-PBS)

    IHC staining of mouse lung using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RALB antibody included
  • IHC staining of human lung cancer using 67094-1-Ig (same clone as 67094-1-PBS)

    IHC staining of human lung cancer using 67094-1-Ig (same clone as 67094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67094-1-Ig (RALB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67094-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RALB antibody included
  • Cytometric bead array standard curve of MP50129-1

    Cytometric bead array standard curve of MP50129-1

    Cytometric bead array standard curve of MP50129-1, RALB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67094-2-PBS. Detection antibody: 67094-1-PBS. Standard:Ag28600. Range: 6.25-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy RALB Ready-To-Use IHC Kit. RALB primary antibody included.

产品信息

67094-1-PBS targets RALB as part of a matched antibody pair:

MP50129-1: 67094-2-PBS capture and 67094-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF, IHC, Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity Human, rat, pig, mouse
Immunogen RALB fusion protein Ag28600 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Affinity
KD=1.82 x 10-10M
KOff=2.54 x 10-6M
KOn=1.40 x 104M
Full Name v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
Synonyms RALB, Ras related protein Ral B
Calculated Molecular Weight 206 aa, 23 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC018163
Gene Symbol RALB
Gene ID (NCBI) 5899
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11234
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.