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验证数据展示

  • WB analysis using 67097-1-Ig (same clone as 67097-1-PBS)

    WB analysis using 67097-1-Ig (same clone as 67097-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • WB analysis of Neuro-2a using 67097-1-Ig (same clone as 67097-1-PBS)

    WB analysis of Neuro-2a using 67097-1-Ig (same clone as 67097-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67097-1-Ig (ELAVL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67097-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50972-1

    Cytometric bead array standard curve of MP50972-1

    Cytometric bead array standard curve of MP50972-1, ELAVL2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67097-1-PBS. Detection antibody: 67097-2-PBS. Standard:Ag28310. Range: 0.391-100 ng/mL.

ELAVL2 Monoclonal antibody, PBS Only (Capture)

ELAVL2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 67097-1-PBS

产品说明书

CloneNo. 2D8C10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, Cytometric bead array, Indirect ELISA

别名

Hu-antigen B, ELAV-like protein 2, ELAV-like neuronal protein 1, ELAVL 2, ELAV2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67097-1-PBS targets ELAVL2 as part of a matched antibody pair:

MP50972-1: 67097-1-PBS capture and 67097-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 ELAVL2 fusion protein Ag28310 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
别名 Hu-antigen B, ELAV-like protein 2, ELAV-like neuronal protein 1, ELAVL 2, ELAV2
计算分子量 39 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC030692
基因名称 ELAVL2
Gene ID (NCBI) 1993
RRIDAB_2882402
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ12926
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR[PMID:18715504,21695151].