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验证数据展示

  • WB analysis using 67111-1-Ig

    WB analysis using 67111-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67111-1-Ig (DLX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67111-1-Ig

    IHC staining of mouse brain using 67111-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67111-1-Ig (DLX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DLX5 Monoclonal antibody

DLX5 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67111-1-Ig

产品说明书

CloneNo. 1C9G1

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

distal less homeobox 5, DLX5, Homeobox protein DLX 5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-12 cells, A375 cells, U-251 cells, THP-1 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67111-1-Ig targets DLX5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原 DLX5 fusion protein Ag17040 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 distal-less homeobox 5
别名 distal less homeobox 5, DLX5, Homeobox protein DLX 5
计算分子量 32 kDa
观测分子量 33-38 kDa
GenBank蛋白编号BC006226
基因名称 DLX5
Gene ID (NCBI) 1749
RRIDAB_2882415
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP56178
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

DLX5 belongs to the DLX family of homeobox transcription factors (Dlx1, Dlx2, Dlx5 and Dlx6), which is expressed in overlapping domains at different stages of cell differentiation in the subpallium and controls differentiation of GABAergic neurons. DLX5 is an immediate early BMP-responsive transcriptional activator that essential for osteoblast differentiation. It can bind to and stimulate the homeodomain-response element of the ALPL and SP7 promoter. DLX5 can form homodimer with DLX2(~34kd) and heterodimer with MSX2(~29kd).

实验方案

Product Specific Protocols
WB protocol for DLX5 antibody 67111-1-IgDownload protocol
IHC protocol for DLX5 antibody 67111-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols