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VEGF-C Monoclonal antibody

VEGF-C Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B11F7

Cat no : 67116-1-Ig

Print datasheet

Synonyms

VEGFC, VEGF C, Vascular endothelial growth factor-related protein, vascular endothelial growth factor C, 2B11F7

验证数据展示

  • WB analysis of MCF-7 using 67116-1-Ig

    WB analysis of MCF-7 using 67116-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 67116-1-Ig

    IF Staining of MCF-7 using 67116-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using VEGFC antibody (67116-1-Ig, Clone: 2B11F7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, T-47D cells, NCI-H1299 cells, A549 cells, LNCaP cells, HT-1080 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy VEGFC Ready-To-Use IHC Kit. VEGFC primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67116-1-Ig targets VEGF-C in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen VEGF-C fusion protein Ag18182 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vascular endothelial growth factor C
Synonyms VEGFC, VEGF C, Vascular endothelial growth factor-related protein, vascular endothelial growth factor C, 2B11F7
Calculated Molecular Weight 419 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC035212
Gene Symbol VEGFC
Gene ID (NCBI) 7424
RRIDAB_2882420
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49767
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for VEGF-C antibody 67116-1-IgDownload protocol
IF protocol for VEGF-C antibody 67116-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Cycle

CircHGS enhances the progression of bladder cancer by regulating the miR-513a-5p/VEGFC axis and activating the AKT/mTOR signaling pathway

Authors - Yi Zhu
View Article