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CoraLite® Plus 488-conjugated VEGF-C Monoclonal antibody

VEGF-C Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B11F7

Cat no : CL488-67116

Print datasheet

Synonyms

VEGFC, VEGF C, Vascular endothelial growth factor-related protein, vascular endothelial growth factor C, 2B11F7

验证数据展示

  • IF Staining of MCF-7 using CL488-67116

    IF Staining of MCF-7 using CL488-67116

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 VEGFC antibody (CL488-67116, Clone: 2B11F7 ) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-67116

    FC experiment of MCF-7 using CL488-67116

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated VEGFC Monoclonal antibody (CL488-67116, Clone:2B11F7)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy VEGFC Ready-To-Use IHC Kit. VEGFC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67116-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67116 targets VEGF-C in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen VEGF-C fusion protein Ag18182 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vascular endothelial growth factor C
Synonyms VEGFC, VEGF C, Vascular endothelial growth factor-related protein, vascular endothelial growth factor C, 2B11F7
Calculated Molecular Weight 419 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC035212
Gene Symbol VEGFC
Gene ID (NCBI) 7424
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49767
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VEGFC is a member of the platelet-derived growth factor / vascular endothelial growth factor (PDGF/VEGF) family. The main function of VEGFC is to promote the growth of lymphatic vessels (lymphangiogenesis). It acts on lymphatic endothelial cells (LECs) primarily via its receptor VEGFR-3 promoting survival, growth, and migration.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 VEGF-C antibody CL488-67116Download protocol
FC protocol for CL Plus 488 VEGF-C antibody CL488-67116Download protocol
Standard Protocols
Click here to view our Standard Protocols