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CD138/Syndecan-1 Monoclonal antibody, PBS Only (Detector)

CD138/Syndecan-1 Monoclonal Antibody for IF, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4F3A8

Cat No : 67155-1-PBS

Print datasheet

Synonyms

CD138, CD138/Syndecan-1, SDC, SDC1, SYND1, syndecan, syndecan 1

验证数据展示

  • IHC staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

    IHC staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (SDC1,CD138 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD138/SDC1 antibody included
  • IHC staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

    IHC staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (SDC1,CD138 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD138/SDC1 antibody included
  • IF Staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

    IF Staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD138/Syndecan-1 antibody (67155-1-Ig, Clone: 4F3A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

    IF Staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD138/Syndecan-1 antibody (67155-1-Ig, Clone: 4F3A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50044-1

    Sandwich ELISA standard curve of MP50044-1

    Sandwich ELISA standard curve of MP50044-1, SDC1,CD138 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60185-1-PBS. Detection antibody: 67155-1-PBS. Standard: Ag17733. Range: 0.188-12 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy CD138/SDC1 Ready-To-Use IHC Kit. CD138/SDC1 primary antibody included.

产品信息

67155-1-PBS targets CD138/Syndecan-1 as part of a matched antibody pair:

MP50044-1: 60185-1-PBS capture and 67155-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications IF, IHC, Sandwich ELISA, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen CD138/Syndecan-1 fusion protein Ag0823 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name syndecan 1
Synonyms CD138, CD138/Syndecan-1, SDC, SDC1, SYND1, syndecan, syndecan 1
Calculated Molecular Weight 32.5 kDa
GenBank Accession NumberBC008765
Gene Symbol SDC1
Gene ID (NCBI) 6382
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18827
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.