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验证数据展示

  • WB analysis using 67183-1-Ig

    WB analysis using 67183-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67183-1-Ig (DMAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 67183-1-Ig

    IF Staining of HEK-293 using 67183-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DMAP1 antibody (67183-1-Ig, Clone: 1A4E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

DMAP1 Monoclonal antibody

DMAP1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67183-1-Ig

产品说明书

CloneNo. 1A4E8

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IF/ICC, ELISA

别名

DKFZp686L09142, DMAP1, DNMAP1, DNMT1 associated protein 1, DNMTAP1, EAF2, FLJ11543, KIAA1425, SWC4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy DMAP1 Ready-To-Use IHC Kit. DMAP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67183-1-Ig targets DMAP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 DMAP1 fusion protein Ag28906 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DNA methyltransferase 1 associated protein 1
别名 DKFZp686L09142, DMAP1, DNMAP1, DNMT1 associated protein 1, DNMTAP1, EAF2, FLJ11543, KIAA1425, SWC4
计算分子量 53 kDa
观测分子量 60 kDa
GenBank蛋白编号BC002855
基因名称 DMAP1
Gene ID (NCBI) 55929
RRIDAB_2882479
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NPF5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DMAP1 (DNA methyltransferase 1-associated protein 1) a component of the NuA4 histone acetyltransferase complex, was originally identified as an interacting molecule with DNA methyltransferase 1 (DNMT1) which co-localizes with PCNA at DNA replication foci. DMAP1 is required for DNA repair and acts against genomic instability. DMAP1 is widely implicated in transcription repression and activation via its interaction with multiple targets including histone deacetylase2 (HDAC2).

实验方案

Product Specific Protocols
WB protocol for DMAP1 antibody 67183-1-IgDownload protocol
IF protocol for DMAP1 antibody 67183-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols