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CoraLite® Plus 488-conjugated DMAP1 Monoclonal antibody

DMAP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A4E8

Cat no : CL488-67183

Print datasheet

Synonyms

EAF2, DNMTAP1, DNMT1-associated protein 1, DNMT1 associated protein 1, DNMAP1

验证数据展示

  • IF Staining of HEK-293 using CL488-67183

    IF Staining of HEK-293 using CL488-67183

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 DMAP1 antibody (CL488-67183, Clone: 1A4E8 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67183

    FC experiment of HeLa using CL488-67183

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DMAP1 (CL488-67183, Clone:1A4E8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DMAP1 Ready-To-Use IHC Kit. DMAP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67183-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67183 targets DMAP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen DMAP1 fusion protein Ag28906 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DNA methyltransferase 1 associated protein 1
Synonyms EAF2, DNMTAP1, DNMT1-associated protein 1, DNMT1 associated protein 1, DNMAP1
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC002855
Gene Symbol DMAP1
Gene ID (NCBI) 55929
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NPF5
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DMAP1 (DNA methyltransferase 1-associated protein 1) a component of the NuA4 histone acetyltransferase complex, was originally identified as an interacting molecule with DNA methyltransferase 1 (DNMT1) which co-localizes with PCNA at DNA replication foci. DMAP1 is required for DNA repair and acts against genomic instability. DMAP1 is widely implicated in transcription repression and activation via its interaction with multiple targets including histone deacetylase2 (HDAC2).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DMAP1 antibody CL488-67183Download protocol
Standard Protocols
Click here to view our Standard Protocols