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验证数据展示

  • IF Staining of HEK-293 using CL488-67183

    IF Staining of HEK-293 using CL488-67183

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 DMAP1 antibody (CL488-67183, Clone: 1A4E8 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67183

    FC experiment of HeLa using CL488-67183

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DMAP1 (CL488-67183, Clone:1A4E8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated DMAP1 Monoclonal antibody

DMAP1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67183

产品说明书

CloneNo. 1A4E8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

EAF2, DNMTAP1, DNMT1-associated protein 1, DNMT1 associated protein 1, DNMAP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DMAP1 Ready-To-Use IHC Kit. DMAP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67183-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67183 targets DMAP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 DMAP1 fusion protein Ag28906 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DNA methyltransferase 1 associated protein 1
别名 EAF2, DNMTAP1, DNMT1-associated protein 1, DNMT1 associated protein 1, DNMAP1
计算分子量 53 kDa
观测分子量 60 kDa
GenBank蛋白编号BC002855
基因名称 DMAP1
Gene ID (NCBI) 55929
RRIDAB_2934526
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NPF5
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DMAP1 (DNA methyltransferase 1-associated protein 1) a component of the NuA4 histone acetyltransferase complex, was originally identified as an interacting molecule with DNA methyltransferase 1 (DNMT1) which co-localizes with PCNA at DNA replication foci. DMAP1 is required for DNA repair and acts against genomic instability. DMAP1 is widely implicated in transcription repression and activation via its interaction with multiple targets including histone deacetylase2 (HDAC2).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DMAP1 antibody CL488-67183Download protocol
Standard Protocols
Click here to view our Standard Protocols