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UBE1 Monoclonal antibody

UBE1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H7E8

Cat no : 67198-1-Ig

Print datasheet

Synonyms

A1S9, A1S9T, A1ST, AMCX1, GXP1, Protein A1S9, SMAX2, UBA1, UBA1A, UBE1, UBE1X, Ubiquitin activating enzyme E1

验证数据展示

  • WB analysis using 67198-1-Ig

    WB analysis using 67198-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67198-1-Ig (UBE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67198-1-Ig

    IHC staining of human colon cancer using 67198-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UBA1 antibody included
  • IHC staining of human colon cancer using 67198-1-Ig

    IHC staining of human colon cancer using 67198-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UBA1 antibody included
  • IF Staining of HeLa using 67198-1-Ig

    IF Staining of HeLa using 67198-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBE1 antibody (67198-1-Ig, Clone: 1H7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HeLa cell, HEK-293 cells, Jurkat cells, HL-60 cells, K-562 cells, HSC-T6 cells, RAW 264.7 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy UBA1 Ready-To-Use IHC Kit. UBA1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

67198-1-Ig targets UBE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen UBE1 fusion protein Ag8920 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ubiquitin-like modifier activating enzyme 1
Synonyms A1S9, A1S9T, A1ST, AMCX1, GXP1, Protein A1S9, SMAX2, UBA1, UBA1A, UBE1, UBE1X, Ubiquitin activating enzyme E1
Calculated Molecular Weight 1058 aa, 118 kDa
Observed Molecular Weight 114-118 kDa
GenBank Accession NumberBC013041
Gene Symbol UBA1
Gene ID (NCBI) 7317
RRIDAB_2882491
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22314
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UBE1 antibody 67198-1-IgDownload protocol
IHC protocol for UBE1 antibody 67198-1-IgDownload protocol
IF protocol for UBE1 antibody 67198-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Biosci Rep

Prognostic value and chemotherapy response prediction of a proliferation essential gene signature in colon cancer

Authors - Jinsheng Liu
View Article