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CoraLite® Plus 488-conjugated UBE1 Monoclonal antibody

UBE1 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H7E8

Cat no : CL488-67198

Print datasheet

Synonyms

A1S9, A1S9T, A1ST, AMCX1, GXP1, Protein A1S9, SMAX2, UBA1, UBA1A, UBE1, UBE1X, Ubiquitin activating enzyme E1

验证数据展示

  • WB analysis using CL488-67198

    WB analysis using CL488-67198

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67198 (UBE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy UBA1 Ready-To-Use IHC Kit. UBA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67198-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67198 targets UBE1 in WB applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB Application Description
Tested Reactivity Human, mouse, rat
Immunogen UBE1 fusion protein Ag8920 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ubiquitin-like modifier activating enzyme 1
Synonyms A1S9, A1S9T, A1ST, AMCX1, GXP1, Protein A1S9, SMAX2, UBA1, UBA1A, UBE1, UBE1X, Ubiquitin activating enzyme E1
Calculated Molecular Weight 1058 aa, 118 kDa
Observed Molecular Weight 114-118 kDa
GenBank Accession NumberBC013041
Gene Symbol UBA1
Gene ID (NCBI) 7317
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22314
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 UBE1 antibody CL488-67198Download protocol
Standard Protocols
Click here to view our Standard Protocols