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LAIR1 Monoclonal antibody

LAIR1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1A5D1

Cat no : 67220-1-Ig

Print datasheet

Synonyms

Leukocyte-associated immunoglobulin-like receptor 1, LAIR-1, LAIR 1, hLAIR1, CD305

验证数据展示

  • WB analysis of THP-1 using 67220-1-Ig

    WB analysis of THP-1 using 67220-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67220-1-Ig (LAIR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 67220-1-Ig

    IHC staining of human tonsillitis using 67220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67220-1-Ig (LAIR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67220-1-Ig

    IHC staining of human tonsillitis using 67220-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67220-1-Ig (LAIR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 67220-1-Ig

    IF Staining of human tonsillitis using 67220-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAIR1 antibody (67220-1-Ig, Clone: 1A5D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 67220-1-Ig

    IF Staining of human tonsillitis using 67220-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAIR1 antibody (67220-1-Ig, Clone: 1A5D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of THP-1 using 67220-1-Ig

    IF Staining of THP-1 using 67220-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using LAIR1 antibody (67220-1-Ig, Clone: 1A5D1 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

经过测试的应用

Positive WB detected inTHP-1 cells, HL-60 cells, TF-1 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inTHP-1 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67220-1-Ig targets LAIR1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen LAIR1 fusion protein Ag14459 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name leukocyte-associated immunoglobulin-like receptor 1
Synonyms Leukocyte-associated immunoglobulin-like receptor 1, LAIR-1, LAIR 1, hLAIR1, CD305
Calculated Molecular Weight 287 aa, 31 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC027899
Gene Symbol LAIR1
Gene ID (NCBI) 3903
RRIDAB_2882511
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6GTX8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) is a transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs (PMID: 9285412). LAIR1 is expressed on the majority of human PBMCs, including NK, T, B, monocytes, and dendritic cells, as well as the majority of thymocytes (PMID: 10229813). It functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of NK cells, B cells and T cells. LAIR1 is a collagen receptor (PMID: 16754721). Tumor-expressed collagens can modulate immune cell function through the inhibitory collagen receptor LAIR1 (PMID: 21955987).

实验方案

Product Specific Protocols
WB protocol for LAIR1 antibody 67220-1-IgDownload protocol
IHC protocol for LAIR1 antibody 67220-1-IgDownload protocol
IF protocol for LAIR1 antibody 67220-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Cancer Med

Development and validation of a leukocyte-associated immunoglobulin-like receptor-1 prognostic signature for lower-grade gliomas.

Authors - Zhansheng Fang
View Article