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ARPC1B Monoclonal antibody

ARPC1B Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C8A1

Cat no : 67320-1-Ig

Print datasheet

Synonyms

ARC41, Arp2/3 complex 41 kDa subunit, ARPC1B, p40 ARC, p41 ARC

验证数据展示

  • WB analysis using 67320-1-Ig

    WB analysis using 67320-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67320-1-Ig (ARPC1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human appendicitis using 67320-1-Ig

    IHC staining of human appendicitis using 67320-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67320-1-Ig (ARPC1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARPC1B antibody included
  • IHC staining of mouse spleen using 67320-1-Ig

    IHC staining of mouse spleen using 67320-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67320-1-Ig (ARPC1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARPC1B antibody included

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, THP-1 cells, rat spleen tissues
Positive IHC detected inhuman appendicitis tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ARPC1B Ready-To-Use IHC Kit. ARPC1B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67320-1-Ig targets ARPC1B in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, rat, mouse
Immunogen ARPC1B fusion protein Ag29095 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name actin related protein 2/3 complex, subunit 1B, 41kDa
Synonyms ARC41, Arp2/3 complex 41 kDa subunit, ARPC1B, p40 ARC, p41 ARC
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC007555
Gene Symbol ARPC1B
Gene ID (NCBI) 10095
RRIDAB_2882580
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15143
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARPC1B also known as ARC41, p41-ARC, or p40-ARC, is one of seven subunits of the human Arp2/3 protein complex, that belongs to the SOP2 family. ARPC1B localizes on centrosomes and has a distinct role in centrosomal homeostasis. ARPC1B is both a physiological activator and substrate of Aurora A kinase, and these interactions help to maintain mitotic integrity in mammalian cells. In recent resaeach, ARPC1B is selected as a candidate prediction marker for sensitivity of Choroidal malignant melanomas to radiotherapy.

实验方案

Product Specific Protocols
WB protocol for ARPC1B antibody 67320-1-IgDownload protocol
IHC protocol for ARPC1B antibody 67320-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols