验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, NCCIT cells, HeLa cells, PC-3 cells, HepG2 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67361-1-Ig targets SPAST in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | human |
| 免疫原 | SPAST fusion protein Ag18866 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | spastin |
| 别名 | ADPSP, FSP2, KIAA1083, SPAST, Spastic paraplegia 4 protein, spastin, SPG4 |
| 计算分子量 | 616 aa, 67 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC150260 |
| 基因名称 | SPAST |
| Gene ID (NCBI) | 6683 |
| RRID | AB_2882613 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UBP0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPAST encodes a microtubule-severing protein called spastin. By severing microtubules into small segments, SPAST participates in the regeneration of neurites. Mutations in the SPAST gene (previously known as SPG4) are the most common causes of hereditary spastic paraplegia (HSP-SPG4). Mammalian cells express at least two spastin isoforms, a less-abundant full-length form (M1-spastin, 68 kDa) and a more highly expressed isoform (M87-spastin, 60 kDa) that lacks the N-terminal 86 amino acids of the full-length protein. Also, there are two additional 64 kDa and 55 kDa isoforms due to alternative splicing of SPG4 exon 4. This antibody recognizes all these isoforms of spastin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPAST antibody 67361-1-Ig | Download protocol |
| IHC protocol for SPAST antibody 67361-1-Ig | Download protocol |
| IF protocol for SPAST antibody 67361-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |