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验证数据展示

  • WB analysis using 67361-1-Ig (same clone as 67361-1-PBS)

    WB analysis using 67361-1-Ig (same clone as 67361-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67361-1-Ig (SPAST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67361-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67361-1-Ig (same clone as 67361-1-PBS)

    IHC staining of mouse brain using 67361-1-Ig (same clone as 67361-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67361-1-Ig (SPAST antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67361-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67361-1-Ig (same clone as 67361-1-PBS)

    IHC staining of mouse brain using 67361-1-Ig (same clone as 67361-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67361-1-Ig (SPAST antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67361-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 67361-1-Ig (same clone as 67361-1-PBS)

    IF Staining of mouse brain using 67361-1-Ig (same clone as 67361-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SPAST antibody (67361-1-Ig, Clone: 1F5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67361-1-PBS in a different storage buffer formulation.

SPAST Monoclonal antibody, PBS Only

SPAST Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67361-1-PBS

产品说明书

CloneNo. 1F5A7

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

ADPSP, FSP2, KIAA1083, SPAST, Spastic paraplegia 4 protein, spastin, SPG4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67361-1-PBS targets SPAST in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 Human, mouse
免疫原 SPAST fusion protein Ag18866 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 spastin
别名 ADPSP, FSP2, KIAA1083, SPAST, Spastic paraplegia 4 protein, spastin, SPG4
计算分子量 616 aa, 67 kDa
观测分子量 55 kDa
GenBank蛋白编号BC150260
基因名称 SPAST
Gene ID (NCBI) 6683
RRIDAB_2882613
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UBP0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPAST encodes a microtubule-severing protein called spastin. By severing microtubules into small segments, SPAST participates in the regeneration of neurites. Mutations in the SPAST gene (previously known as SPG4) are the most common causes of hereditary spastic paraplegia (HSP-SPG4). Mammalian cells express at least two spastin isoforms, a less-abundant full-length form (M1-spastin, 68 kDa) and a more highly expressed isoform (M87-spastin, 60 kDa) that lacks the N-terminal 86 amino acids of the full-length protein. Also, there are two additional 64 kDa and 55 kDa isoforms due to alternative splicing of SPG4 exon 4. This antibody recognizes all these isoforms of spastin.