• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67392-1-Ig

    WB analysis using 67392-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67392-1-Ig (PPP4R1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of human liver cancer using 67392-1-Ig

    IF Staining of human liver cancer using 67392-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using PPP4R1 antibody (67392-1-Ig, Clone: 3D12G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of human liver cancer using 67392-1-Ig

    IHC staining of human liver cancer using 67392-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67392-1-Ig (PPP4R1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67392-1-Ig

    IHC staining of human liver cancer using 67392-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67392-1-Ig (PPP4R1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 67392-1-Ig

    FC experiment of HeLa using 67392-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PPP4R1 (67392-1-Ig, Clone:3D12G12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

PPP4R1 Monoclonal antibody

PPP4R1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 67392-1-Ig

产品说明书

CloneNo. 3D12G12
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, FC (Intra), ELISA and More (1)

别名

Serine/threonine-protein phosphatase 4 regulatory subunit 1, PP4R1, PP4(Rmeg), MEG1, 3D12G12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue, mouse liver tissue
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67392-1-Ig targets PPP4R1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 PPP4R1 fusion protein Ag26750 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein phosphatase 4, regulatory subunit 1
别名 Serine/threonine-protein phosphatase 4 regulatory subunit 1, PP4R1, PP4(Rmeg), MEG1, 3D12G12
计算分子量 107 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC060829
基因名称 PPP4R1
Gene ID (NCBI) 9989
RRIDAB_2882636
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8TF05
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPP4R1 antibody 67392-1-IgDownload protocol
IHC protocol for PPP4R1 antibody 67392-1-IgDownload protocol
IF protocol for PPP4R1 antibody 67392-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Transl Cancer Res

The diagnostic, prognostic, drug sensitivity and ceRNA internet of PPP4R1 in liver hepatocellular carcinoma (LIHC)

Authors - Xiaowei Li
View Article