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验证数据展示

  • IF Staining of mouse liver using CL594-67392

    IF Staining of mouse liver using CL594-67392

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite®594 PPP4R1 antibody (CL594-67392, Clone: 3D12G12 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-67392

    FC experiment of HeLa using CL594-67392

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PPP4R1 (CL594-67392, Clone:3D12G12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated PPP4R1 Monoclonal antibody

PPP4R1 Monoclonal Antibody for IF-P, FC (Intra)
Cat No. CL594-67392

Print datasheet

CloneNo. 3D12G12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Synonyms

Serine/threonine-protein phosphatase 4 regulatory subunit 1, PP4R1, PP4(Rmeg), MEG1, 3D12G12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse liver tissue
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67392-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67392 targets PPP4R1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen PPP4R1 fusion protein Ag26750 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein phosphatase 4, regulatory subunit 1
Synonyms Serine/threonine-protein phosphatase 4 regulatory subunit 1, PP4R1, PP4(Rmeg), MEG1, 3D12G12
Calculated Molecular Weight 107 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC060829
Gene Symbol PPP4R1
Gene ID (NCBI) 9989
RRIDAB_3673518
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8TF05
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 PPP4R1 antibody CL594-67392Download protocol
FC protocol for CL594 PPP4R1 antibody CL594-67392Download protocol
Standard Protocols
Click here to view our Standard Protocols