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验证数据展示

  • WB analysis using 67427-1-Ig

    WB analysis using 67427-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67427-1-Ig (KLK11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67427-1-Ig

    IHC staining of human breast cancer using 67427-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67427-1-Ig (KLK11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KLK11 antibody included
  • IHC staining of human breast cancer using 67427-1-Ig

    IHC staining of human breast cancer using 67427-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67427-1-Ig (KLK11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KLK11 antibody included
  • IF Staining of human breast cancer using 67427-1-Ig

    IF Staining of human breast cancer using 67427-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using KLK11 antibody (67427-1-Ig, Clone: 1E2B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human breast cancer using 67427-1-Ig

    IF Staining of human breast cancer using 67427-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using KLK11 antibody (67427-1-Ig, Clone: 1E2B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 67427-1-Ig

    WB analysis using 67427-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67427-1-Ig (KLK11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

KLK11 Monoclonal antibody

KLK11 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67427-1-Ig

产品说明书

CloneNo. 1E2B4
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, ELISA

别名

Kallikrein 11, Kallikrein-11 inactive chain 1, Kallikrein-11, Kallikrein11, EC:3.4.21.-

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTT cells, DU 145 cells, pig brain tissue, LNCaP cells, PC-3 cells, Jurkat cells, rat brain tissue, A431 cells, HaCaT cells, HT-29 cells, SW 1990 cells, mouse brain tissue, rat skin tissue
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy KLK11 Ready-To-Use IHC Kit. KLK11 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67427-1-Ig targets KLK11 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat, pig
文献引用反应性human
免疫原 KLK11 fusion protein Ag29895 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 kallikrein-related peptidase 11
别名 Kallikrein 11, Kallikrein-11 inactive chain 1, Kallikrein-11, Kallikrein11, EC:3.4.21.-
计算分子量 250 aa, 27 kDa
观测分子量 30-40 kDa
GenBank蛋白编号BC022068
基因名称 KLK11
Gene ID (NCBI) 11012
RRIDAB_2882666
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UBX7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.

实验方案

Product Specific Protocols
WB protocol for KLK11 antibody 67427-1-IgDownload protocol
IHC protocol for KLK11 antibody 67427-1-IgDownload protocol
IF protocol for KLK11 antibody 67427-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Bioenerg Biomembr

KLK11 acts as a tumor-inhibitor in laryngeal squamous cell carcinoma through the inactivation of Akt/Wnt/β-catenin signaling.

Authors - Ruimin Zhao
View Article
humanWB,IHC

Carcinogenesis

miR-1304 targets KLK11 to regulate gastric cancer cell proliferation through the mTOR signaling pathway

Authors - Yi Ding
View Article