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验证数据展示

  • FC experiment of HepG2 using CL594-67427

    FC experiment of HepG2 using CL594-67427

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human KLK11 (CL594-67427, Clone:1E2B4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated KLK11 Monoclonal antibody

KLK11 Monoclonal Antibody for FC (Intra)
Cat No. CL594-67427

Print datasheet

CloneNo. 1E2B4

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Rat

Applications

FC (Intra)

Synonyms

Kallikrein 11, KLK11, PRSS20, Trypsin-like protease, UNQ649/PRO1279, Kallikrein11, Kallikrein-11, Kallikrein-11 inactive chain 1, Kallikrein-11 inactive chain 2, KLK 11, 1E2B4, EC:3.4.21.-

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KLK11 Ready-To-Use IHC Kit. KLK11 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67427-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67427 targets KLK11 in FC (Intra) applications and shows reactivity with Human, Pig, Rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, Pig, Rat
Immunogen KLK11 fusion protein Ag29895 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name kallikrein-related peptidase 11
Synonyms Kallikrein 11, KLK11, PRSS20, Trypsin-like protease, UNQ649/PRO1279, Kallikrein11, Kallikrein-11, Kallikrein-11 inactive chain 1, Kallikrein-11 inactive chain 2, KLK 11, 1E2B4, EC:3.4.21.-
Calculated Molecular Weight 250 aa, 27 kDa
Observed Molecular Weight 30-40 kDa
GenBank Accession NumberBC022068
Gene Symbol KLK11
Gene ID (NCBI) 11012
RRIDAB_3084765
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UBX7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.

实验方案

Product Specific Protocols
FC protocol for CL594 KLK11 antibody CL594-67427Download protocol
Standard Protocols
Click here to view our Standard Protocols