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验证数据展示

  • FC experiment of HepG2 using CL594-67427

    FC experiment of HepG2 using CL594-67427

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human KLK11 (CL594-67427, Clone:1E2B4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated KLK11 Monoclonal antibody

KLK11 Monoclonal Antibody for FC (Intra)
Cat No. CL594-67427

产品说明书

CloneNo. 1E2B4

宿主/亚型

Mouse / IgG1

种属反应性

Human, Pig, Rat

应用

FC (Intra)

别名

Kallikrein 11, KLK11, PRSS20, Trypsin-like protease, UNQ649/PRO1279, Kallikrein11, Kallikrein-11, Kallikrein-11 inactive chain 1, Kallikrein-11 inactive chain 2, KLK 11, 1E2B4, EC:3.4.21.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KLK11 Ready-To-Use IHC Kit. KLK11 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67427-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67427 targets KLK11 in FC (Intra) applications and shows reactivity with Human, Pig, Rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Pig, Rat
免疫原 KLK11 fusion protein Ag29895 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 kallikrein-related peptidase 11
别名 Kallikrein 11, KLK11, PRSS20, Trypsin-like protease, UNQ649/PRO1279, Kallikrein11, Kallikrein-11, Kallikrein-11 inactive chain 1, Kallikrein-11 inactive chain 2, KLK 11, 1E2B4, EC:3.4.21.-
计算分子量 250 aa, 27 kDa
观测分子量 30-40 kDa
GenBank蛋白编号BC022068
基因名称 KLK11
Gene ID (NCBI) 11012
RRIDAB_3084765
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UBX7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.

实验方案

Product Specific Protocols
FC protocol for CL594 KLK11 antibody CL594-67427Download protocol
Standard Protocols
Click here to view our Standard Protocols