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验证数据展示

  • WB analysis of MCF-7 using 67440-1-Ig

    WB analysis of MCF-7 using 67440-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67440-1-Ig (PPM1D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67440-1-Ig

    IHC staining of human colon cancer using 67440-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67440-1-Ig (PPM1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPM1D antibody included
  • IHC staining of human colon cancer using 67440-1-Ig

    IHC staining of human colon cancer using 67440-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67440-1-Ig (PPM1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPM1D antibody included
  • IF Staining of HepG2 using 67440-1-Ig

    IF Staining of HepG2 using 67440-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67440-1-Ig (PPM1D antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PPM1D Monoclonal antibody

PPM1D Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67440-1-Ig

产品说明书

CloneNo. 2D6D5

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IHC, IF/ICC, ELISA

别名

PP2C DELTA, PPM1D, Protein phosphatase 1D, WIP1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293 cells, U2OS cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PPM1D Ready-To-Use IHC Kit. PPM1D primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67440-1-Ig targets PPM1D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 PPM1D fusion protein Ag24761 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein phosphatase 1D magnesium-dependent, delta isoform
别名 PP2C DELTA, PPM1D, Protein phosphatase 1D, WIP1
观测分子量 67 kDa, 47 kDa
GenBank蛋白编号BC060877
基因名称 PPM1D
Gene ID (NCBI) 8493
RRIDAB_2882676
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO15297
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPM1D (also known as PP2Cd and WIP1) is a member of the PPM family, which is characterized by Mg2+/Mn2+-dependent activity and relative insensitivity to okadaic acid. The PPM1D gene is aberrantly amplified in a range of common cancers and encodes a protein phosphatase that is a potential therapeutic target (PMID: 17700519). This antibody can be detected two bands of 47 and 67 Kda.

实验方案

Product Specific Protocols
WB protocol for PPM1D antibody 67440-1-IgDownload protocol
IHC protocol for PPM1D antibody 67440-1-IgDownload protocol
IF protocol for PPM1D antibody 67440-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols