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PPM1D Monoclonal antibody, PBS Only

PPM1D Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D6D5

Cat no : 67440-1-PBS

Print datasheet

Synonyms

PP2C DELTA, PPM1D, Protein phosphatase 1D, WIP1

验证数据展示

  • WB analysis of MCF-7 using 67440-1-Ig (same clone as 67440-1-PBS)

    WB analysis of MCF-7 using 67440-1-Ig (same clone as 67440-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67440-1-Ig (PPM1D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67440-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67440-1-Ig (same clone as 67440-1-PBS)

    IHC staining of human colon cancer using 67440-1-Ig (same clone as 67440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67440-1-Ig (PPM1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67440-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPM1D antibody included
  • IHC staining of human colon cancer using 67440-1-Ig (same clone as 67440-1-PBS)

    IHC staining of human colon cancer using 67440-1-Ig (same clone as 67440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67440-1-Ig (PPM1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67440-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPM1D antibody included
  • IF Staining of HepG2 using 67440-1-Ig (same clone as 67440-1-PBS)

    IF Staining of HepG2 using 67440-1-Ig (same clone as 67440-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67440-1-Ig (PPM1D antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67440-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PPM1D Ready-To-Use IHC Kit. PPM1D primary antibody included.

产品信息

67440-1-PBS targets PPM1D in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen PPM1D fusion protein Ag24761 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein phosphatase 1D magnesium-dependent, delta isoform
Synonyms PP2C DELTA, PPM1D, Protein phosphatase 1D, WIP1
Observed Molecular Weight 67 kDa, 47 kDa
GenBank Accession NumberBC060877
Gene Symbol PPM1D
Gene ID (NCBI) 8493
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15297
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPM1D (also known as PP2Cd and WIP1) is a member of the PPM family, which is characterized by Mg2+/Mn2+-dependent activity and relative insensitivity to okadaic acid. The PPM1D gene is aberrantly amplified in a range of common cancers and encodes a protein phosphatase that is a potential therapeutic target (PMID: 17700519). This antibody can be detected two bands of 47 and 67 Kda.