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验证数据展示

  • WB analysis of MCF-7 using 67440-1-Ig (same clone as 67440-1-PBS)

    WB analysis of MCF-7 using 67440-1-Ig (same clone as 67440-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67440-1-Ig (PPM1D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67440-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67440-1-Ig (same clone as 67440-1-PBS)

    IHC staining of human colon cancer using 67440-1-Ig (same clone as 67440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67440-1-Ig (PPM1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67440-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPM1D antibody included
  • IHC staining of human colon cancer using 67440-1-Ig (same clone as 67440-1-PBS)

    IHC staining of human colon cancer using 67440-1-Ig (same clone as 67440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67440-1-Ig (PPM1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67440-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPM1D antibody included
  • IF Staining of HepG2 using 67440-1-Ig (same clone as 67440-1-PBS)

    IF Staining of HepG2 using 67440-1-Ig (same clone as 67440-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67440-1-Ig (PPM1D antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67440-1-PBS in a different storage buffer formulation.

PPM1D Monoclonal antibody, PBS Only

PPM1D Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67440-1-PBS

Print datasheet

CloneNo. 2D6D5

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PP2C DELTA, PPM1D, Protein phosphatase 1D, WIP1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PPM1D Ready-To-Use IHC Kit. PPM1D primary antibody included.

产品信息

67440-1-PBS targets PPM1D in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen PPM1D fusion protein Ag24761 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein phosphatase 1D magnesium-dependent, delta isoform
Synonyms PP2C DELTA, PPM1D, Protein phosphatase 1D, WIP1
Observed Molecular Weight 67 kDa, 47 kDa
GenBank Accession NumberBC060877
Gene Symbol PPM1D
Gene ID (NCBI) 8493
RRIDAB_2882676
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15297
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPM1D (also known as PP2Cd and WIP1) is a member of the PPM family, which is characterized by Mg2+/Mn2+-dependent activity and relative insensitivity to okadaic acid. The PPM1D gene is aberrantly amplified in a range of common cancers and encodes a protein phosphatase that is a potential therapeutic target (PMID: 17700519). This antibody can be detected two bands of 47 and 67 Kda.