验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, HEK-293 cells, U2OS cells, pig brain tissue, rat brain tissue, mouse brain tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67440-1-Ig targets PPM1D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | Human, Mouse, Rat, Pig |
免疫原 | PPM1D fusion protein Ag24761 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | protein phosphatase 1D magnesium-dependent, delta isoform |
别名 | PP2C DELTA, PPM1D, Protein phosphatase 1D, WIP1 |
观测分子量 | 67 kDa, 47 kDa |
GenBank蛋白编号 | BC060877 |
基因名称 | PPM1D |
Gene ID (NCBI) | 8493 |
RRID | AB_2882676 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | O15297 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPM1D (also known as PP2Cd and WIP1) is a member of the PPM family, which is characterized by Mg2+/Mn2+-dependent activity and relative insensitivity to okadaic acid. The PPM1D gene is aberrantly amplified in a range of common cancers and encodes a protein phosphatase that is a potential therapeutic target (PMID: 17700519). This antibody can be detected two bands of 47 and 67 Kda.
实验方案
Product Specific Protocols | |
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WB protocol for PPM1D antibody 67440-1-Ig | Download protocol |
IHC protocol for PPM1D antibody 67440-1-Ig | Download protocol |
IF protocol for PPM1D antibody 67440-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |