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  • Featured Product
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CEP55 Monoclonal antibody

CEP55 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D11F7

Cat no : 67460-1-Ig

Print datasheet

Synonyms

C10orf3, centrosomal protein 55kDa, Centrosomal protein of 55 kDa, CEP55, FLJ10540, Up regulated in colon cancer 6, URCC6

验证数据展示

  • WB analysis using 67460-1-Ig

    WB analysis using 67460-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67460-1-Ig (CEP55 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67460-1-Ig

    WB analysis using 67460-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67460-1-Ig (CEP55 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67460-1-Ig

    WB analysis of HeLa using 67460-1-Ig

    WB result of CEP55 antibody (67460-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CEP55 transfected HeLa cells.

  • IF Staining of HeLa using 67460-1-Ig

    IF Staining of HeLa using 67460-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67460-1-Ig (CEP55 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 67460-1-Ig

    IF Staining of HeLa using 67460-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CEP55 antibody (67460-1-Ig, Clone: 2D11F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, mouse testis tissue
Positive IF/ICC detected inHeLa cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67460-1-Ig targets CEP55 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen CEP55 fusion protein Ag29995 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name centrosomal protein 55kDa
Synonyms C10orf3, centrosomal protein 55kDa, Centrosomal protein of 55 kDa, CEP55, FLJ10540, Up regulated in colon cancer 6, URCC6
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 47-55 kDa
GenBank Accession NumberBC008947
Gene Symbol CEP55
Gene ID (NCBI) 55165
RRIDAB_2882690
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ53EZ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP55, also named as C10orf3 and URCC6, is a 55 kDa protein which located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. It plays a role in mitotic exit and cytokinesis.CEP55 has two isoforms with the MW of 47 and 55 kDa.

实验方案

Product Specific Protocols
WB protocol for CEP55 antibody 67460-1-IgDownload protocol
IF protocol for CEP55 antibody 67460-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols