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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 67460-1-Ig (same clone as 67460-1-PBS)

    WB analysis using 67460-1-Ig (same clone as 67460-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67460-1-Ig (CEP55 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

  • WB analysis using 67460-1-Ig (same clone as 67460-1-PBS)

    WB analysis using 67460-1-Ig (same clone as 67460-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67460-1-Ig (CEP55 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67460-1-Ig (same clone as 67460-1-PBS)

    WB analysis of HeLa using 67460-1-Ig (same clone as 67460-1-PBS)

    WB result of CEP55 antibody (67460-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CEP55 transfected HeLa cells. This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67460-1-Ig (same clone as 67460-1-PBS)

    IF Staining of HeLa using 67460-1-Ig (same clone as 67460-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67460-1-Ig (CEP55 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67460-1-Ig (same clone as 67460-1-PBS)

    IF Staining of HeLa using 67460-1-Ig (same clone as 67460-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CEP55 antibody (67460-1-Ig, Clone: 2D11F7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse adrenal gland using 67460-1-Ig (same clone as 67460-1-PBS)

    IHC staining of mouse adrenal gland using 67460-1-Ig (same clone as 67460-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 67460-1-Ig (CEP55 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CEP55 antibody included
  • IHC staining of mouse adrenal gland using 67460-1-Ig (same clone as 67460-1-PBS)

    IHC staining of mouse adrenal gland using 67460-1-Ig (same clone as 67460-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 67460-1-Ig (CEP55 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67460-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CEP55 antibody included
  • Featured Product
  • KD/KO验证

CEP55 Monoclonal antibody, PBS Only

CEP55 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67460-1-PBS

产品说明书

CloneNo. 2D11F7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Up-regulated in colon cancer 6, Up regulated in colon cancer 6, Centrosomal protein of 55 kDa, centrosomal protein 55kDa, C10orf3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CEP55 Ready-To-Use IHC Kit. CEP55 primary antibody included.

产品信息

67460-1-PBS targets CEP55 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 CEP55 fusion protein Ag29995 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 centrosomal protein 55kDa
别名 Up-regulated in colon cancer 6, Up regulated in colon cancer 6, Centrosomal protein of 55 kDa, centrosomal protein 55kDa, C10orf3
计算分子量 54 kDa
观测分子量 47-55 kDa
GenBank蛋白编号BC008947
基因名称 CEP55
Gene ID (NCBI) 55165
RRIDAB_2882690
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ53EZ4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CEP55, also named as C10orf3 and URCC6, is a 55 kDa protein which located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. It plays a role in mitotic exit and cytokinesis.CEP55 has two isoforms with the MW of 47 and 55 kDa.