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验证数据展示

  • WB analysis of HEK-293 using 67463-1-Ig

    WB analysis of HEK-293 using 67463-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67463-1-Ig (ZNF746 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

ZNF746 Monoclonal antibody

ZNF746 Monoclonal Antibody for WB, ELISA
Cat No. 67463-1-Ig

产品说明书

CloneNo. 3A12C5

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

FLJ31413, PARIS, zinc finger protein 746, ZNF746

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, LNCaP cells
Planning an IHC experiment? We recommend our IHCeasy ZNF746 Ready-To-Use IHC Kit. ZNF746 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67463-1-Ig targets ZNF746 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 ZNF746 fusion protein Ag21582 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 zinc finger protein 746
别名 FLJ31413, PARIS, zinc finger protein 746, ZNF746
计算分子量 644 aa, 69 kDa
观测分子量85-90 kDa
GenBank蛋白编号BC068505
基因名称 ZNF746
Gene ID (NCBI) 155061
RRIDAB_2918491
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ6NUN9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF746, also named as PARIS, which belongs to the krueppel C2H2-type zinc-finger protein family. ZNF746 mainly localizes to the cytoplasm and probably translocates to the nucleus to repress selected genes. ZNF746 as a transcription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death. ZNF746 may act as a downstream effector of PRKN and contribute to neurodegeneration in Parkinson disease cases caused by defects in PRKN: its accumulation due to the absence of PRKN, followed by up-regulation of PPARGC1A, could lead to the selective loss of dopamine neurons in the substantia nigra(PMID: 28122242). The calculated molecular weight of ZNF746 is 69 kDa, but Phosphorylation of ZNF746 is about 80-85 kDa (PMID: 28122242).

实验方案

Product Specific Protocols
WB protocol for ZNF746 antibody 67463-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols