验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, HSC-T6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67477-1-Ig targets ERAP2 in WB, ELISA applications and shows reactivity with Human, Rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, Rat |
| 文献引用反应性 | human |
| 免疫原 | ERAP2 fusion protein Ag30061 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | endoplasmic reticulum aminopeptidase 2 |
| 别名 | ERAP2, FLJ23633, FLJ23701, FLJ23807, L RAP, LRAP |
| 计算分子量 | 110 kDa |
| 观测分子量 | 110-120 kDa |
| GenBank蛋白编号 | BC065240 |
| 基因名称 | ERAP2 |
| Gene ID (NCBI) | 64167 |
| RRID | AB_2882704 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q6P179 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ERAP2(Endoplasmic reticulum aminopeptidase 2) is also named as LRAP(Leukocyte-derived arginine aminopeptidase). It plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. ERAP2 can form heterodimers with ERAP1(PMID:15908954). Defects in the expression of ERAP2 may cause improper antigen processing, possibly leading to favor tumor escape from the immune surveillance. ERAP2 has the calculated molecular mass of 105-110 kDa, and apparent molecular mass of 120 kDa duo to the glycosylation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ERAP2 antibody 67477-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
