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验证数据展示

  • WB analysis of BXPC-3 using 67485-1-Ig

    WB analysis of BXPC-3 using 67485-1-Ig

    BXPC-3 cells were subjected to SDS PAGE followed by western blot with 67485-1-Ig (S100P antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 67485-1-Ig

    IHC staining of human pancreas cancer using 67485-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100P antibody included
  • IHC staining of human pancreas cancer using 67485-1-Ig

    IHC staining of human pancreas cancer using 67485-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with S100P antibody included
  • IF Staining of HeLa using 67485-1-Ig

    IF Staining of HeLa using 67485-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using S100P antibody (67485-1-Ig, Clone: 1G6C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

S100P Monoclonal antibody

S100P Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67485-1-Ig

产品说明书

CloneNo. 1G6C3

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

S100 calcium binding protein P, Protein S100-P, Protein S100-E, Protein S100 P, Migration-inducing gene 9 protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBXPC-3 cells
Positive IHC detected inhuman pancreas cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy S100P Ready-To-Use IHC Kit. S100P primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:4000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67485-1-Ig targets S100P in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 S100P fusion protein Ag19115 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 S100 calcium binding protein P
别名 S100 calcium binding protein P, Protein S100-P, Protein S100-E, Protein S100 P, Migration-inducing gene 9 protein
计算分子量 95 aa, 11 kDa
观测分子量 10 KDa
GenBank蛋白编号BC006819
基因名称 S100P
Gene ID (NCBI) 6286
RRIDAB_2882712
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25815
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100P protein is a relatively small (95 amino acid) isoform of the S100 protein family that was first isolated from human placenta. Overexpression of S100P has been detected in several cancers such as breast, colon, prostate, pancreatic and lung carcinomas, and the protein has been functionally implicated in carcinogenic processes. S100P protein is widely expressed in both normal and neoplastic tissues. It clearly shows ectopic expression in some cancers. Based on the high expression in certain tumors, S100P could represent a potential target for novel diagnostic and therapeutic applications.

实验方案

Product Specific Protocols
WB protocol for S100P antibody 67485-1-IgDownload protocol
IHC protocol for S100P antibody 67485-1-IgDownload protocol
IF protocol for S100P antibody 67485-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols