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S100P Monoclonal antibody, PBS Only (Capture)

S100P Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G6C3

Cat no : 67485-1-PBS

Print datasheet

Synonyms

S100 calcium binding protein P, Protein S100-P, Protein S100-E, Protein S100 P, Migration-inducing gene 9 protein

验证数据展示

  • WB analysis of BXPC-3 using 67485-1-Ig (same clone as 67485-1-PBS)

    WB analysis of BXPC-3 using 67485-1-Ig (same clone as 67485-1-PBS)

    BXPC-3 cells were subjected to SDS PAGE followed by western blot with 67485-1-Ig (S100P antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67485-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 67485-1-Ig (same clone as 67485-1-PBS)

    IHC staining of human pancreas cancer using 67485-1-Ig (same clone as 67485-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67485-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100P antibody included
  • IF Staining of HeLa using 67485-1-Ig (same clone as 67485-1-PBS)

    IF Staining of HeLa using 67485-1-Ig (same clone as 67485-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using S100P antibody (67485-1-Ig, Clone: 1G6C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67485-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51168-2

    Cytometric bead array standard curve of MP51168-2

    Cytometric bead array standard curve of MP51168-2, S100P Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67485-1-PBS. Detection antibody: 67485-4-PBS. Standard:Ag19115. Range: 6.25-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy S100P Ready-To-Use IHC Kit. S100P primary antibody included.

产品信息

67485-1-PBS targets S100P as part of a matched antibody pair:

MP51168-2: 67485-1-PBS capture and 67485-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen S100P fusion protein Ag19115 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name S100 calcium binding protein P
Synonyms S100 calcium binding protein P, Protein S100-P, Protein S100-E, Protein S100 P, Migration-inducing gene 9 protein
Calculated Molecular Weight 95 aa, 11 kDa
Observed Molecular Weight 10 KDa
GenBank Accession NumberBC006819
Gene Symbol S100P
Gene ID (NCBI) 6286
RRIDAB_2882712
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25815
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

S100P protein is a relatively small (95 amino acid) isoform of the S100 protein family that was first isolated from human placenta. Overexpression of S100P has been detected in several cancers such as breast, colon, prostate, pancreatic and lung carcinomas, and the protein has been functionally implicated in carcinogenic processes. S100P protein is widely expressed in both normal and neoplastic tissues. It clearly shows ectopic expression in some cancers. Based on the high expression in certain tumors, S100P could represent a potential target for novel diagnostic and therapeutic applications.