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AMT Monoclonal antibody

AMT Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3E7D4

Cat no : 67532-1-Ig

Print datasheet

Synonyms

aminomethyltransferase, AMT, GCE, GCST, GCVT, NKH

验证数据展示

  • WB analysis of pig liver using 67532-1-Ig

    WB analysis of pig liver using 67532-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67532-1-Ig

    IHC staining of human liver cancer using 67532-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67532-1-Ig (AMT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67532-1-Ig

    IF Staining of HepG2 using 67532-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AMT antibody (67532-1-Ig, Clone: 3E7D4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

经过测试的应用

Positive WB detected inpig liver tissue, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67532-1-Ig targets AMT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat, pig
Cited Reactivityhuman
Immunogen AMT fusion protein Ag26644 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aminomethyltransferase
Synonyms aminomethyltransferase, AMT, GCE, GCST, GCVT, NKH
Calculated Molecular Weight 44 kDa
Observed Molecular Weight37-44 kDa
GenBank Accession NumberBC007546
Gene Symbol AMT
Gene ID (NCBI) 275
RRIDAB_2882751
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP48728
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AMT antibody 67532-1-IgDownload protocol
IHC protocol for AMT antibody 67532-1-IgDownload protocol
IF protocol for AMT antibody 67532-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

Multiomic landscape of immune pathogenesis in Kimura's disease

Authors - Xunyao Wu
View Article