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AMT Monoclonal antibody, PBS Only (Detector)

AMT Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E7D4

Cat No : 67532-1-PBS

Print datasheet

Synonyms

GCST, GCVT, EC:2.1.2.10, aminomethyltransferase, 3E7D4

验证数据展示

  • WB analysis of pig liver using 67532-1-Ig (same clone as 67532-1-PBS)

    WB analysis of pig liver using 67532-1-Ig (same clone as 67532-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67532-1-Ig (same clone as 67532-1-PBS)

    IHC staining of human liver cancer using 67532-1-Ig (same clone as 67532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67532-1-Ig (AMT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67532-1-Ig (same clone as 67532-1-PBS)

    IF Staining of HepG2 using 67532-1-Ig (same clone as 67532-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AMT antibody (67532-1-Ig, Clone: 3E7D4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50403-2

    Cytometric bead array standard curve of MP50403-2

    Cytometric bead array standard curve of MP50403-2, AMT Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67532-3-PBS. Detection antibody: 67532-1-PBS. Standard:Ag26644. Range: 3.125-100 ng/mL.

产品信息

67532-1-PBS targets AMT as part of a matched antibody pair:

MP50403-2: 67532-3-PBS capture and 67532-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen AMT fusion protein Ag26644 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aminomethyltransferase
Synonyms GCST, GCVT, EC:2.1.2.10, aminomethyltransferase, 3E7D4
Calculated Molecular Weight 44 kDa
Observed Molecular Weight37-44 kDa
GenBank Accession NumberBC007546
Gene Symbol AMT
Gene ID (NCBI) 275
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP48728
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C