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验证数据展示

  • WB analysis of pig liver using 67532-1-Ig (same clone as 67532-1-PBS)

    WB analysis of pig liver using 67532-1-Ig (same clone as 67532-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67532-1-Ig (AMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67532-1-Ig (same clone as 67532-1-PBS)

    IHC staining of human liver cancer using 67532-1-Ig (same clone as 67532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67532-1-Ig (AMT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67532-1-Ig (same clone as 67532-1-PBS)

    IF Staining of HepG2 using 67532-1-Ig (same clone as 67532-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AMT antibody (67532-1-Ig, Clone: 3E7D4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67532-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50403-2

    Cytometric bead array standard curve of MP50403-2

    Cytometric bead array standard curve of MP50403-2, AMT Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67532-3-PBS. Detection antibody: 67532-1-PBS. Standard:Ag26644. Range: 3.125-100 ng/mL.

AMT Monoclonal antibody, PBS Only (Detector)

AMT Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67532-1-PBS

产品说明书

CloneNo. 3E7D4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

GCST, GCVT, EC:2.1.2.10, aminomethyltransferase, 3E7D4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67532-1-PBS targets AMT as part of a matched antibody pair:

MP50403-2: 67532-3-PBS capture and 67532-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 AMT fusion protein Ag26644 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 aminomethyltransferase
别名 GCST, GCVT, EC:2.1.2.10, aminomethyltransferase, 3E7D4
计算分子量 44 kDa
观测分子量37-44 kDa
GenBank蛋白编号BC007546
基因名称 AMT
Gene ID (NCBI) 275
RRIDAB_2882751
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP48728
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C